Nucleic Acid Extraction Kit (A02)
Mfundo yodziwira
Pambuyo potulutsa DNA ya genomic pogawanitsa maselo okhala ndi lysis buffer, mkanda wa maginito ukhoza kumangirira ku genomic DNA mu zitsanzo.Zonyansa zochepa zomwe zimatengedwa ndi mkanda wa maginito zitha kuchotsedwa ndi chotchinga chotsuka.Mu TE, mkanda wa maginito ukhoza kumasula DNA ya boundgenome, kupeza DNA yapamwamba kwambiri ya genome.Njirayi ndi yophweka komanso yofulumira ndipo khalidwe la DNA lotengedwa ndilopamwamba, lomwe lingakwaniritse zofunikira kuti azindikire DNA methylation.Pakadali pano, zida zopangira zida zotengera mkanda wa maginito zitha kugwirizanirana ndi kutulutsa kwa nucleic acid, kukwaniritsa ntchito zapamwamba kwambiri zochotsa nucleic acid.
Zigawo zazikulu za reagent
Magawo akuwonetsedwa mu tebulo 1:
Table 1 Reagent Zigawo ndi Kutsegula
| Dzina lachigawo | Zigawo zazikulu | Kukula (48) | Kukula (200) |
| 1. Digestion Buffer A | Tris, SDS | 15.8 mL / botolo | 66mL / botolo |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 mL / botolo | 66mL / botolo |
| 3. Sambani Buffer A | NaCl, Tris | 11 mL / botolo | 44 ml / botolo |
| 4. Sambani Buffer B | NaCl, Tris | 13 mL / botolo | 26.5mL/botolo *2 |
| 5. TE | Tris, EDTA | 12 ml / botolo | 44 ml / botolo |
| 6. Protease K solution | Protease K | 1.1mL / chidutswa | 4.4mL / chidutswa |
| 7. Kuyimitsidwa kwa maginito 2 | Maginito mikanda | 0.5mL / chidutswa | 2.2mL / chidutswa |
| 8. Malangizo ochotsa nucleic acid reagents | / | 1 kopi | 1 kopi |
Zomwe zimafunikira pakuchotsa kwa nucleic acid, koma sizinaphatikizidwe mu zida:
1. Reagent: Anhydrous ethanol, isopropanol, ndi PBS;
2. Zowonongeka: 50mL centrifuge chubu ndi 1.5mL EP chubu;
3. Zida: Kusamba madzi, pipettes, alumali maginito, centrifuge, 96-chitsime mbale (zodziwikiratu), basi nucleic asidi m'zigawo zida (zodziwikiratu).
Zambiri zoyambira
Zitsanzo zofunika:
1.Kuzindikira kudzamalizidwa pansi pa kusungidwa kwa masiku 7 kwa kutentha kozungulira pambuyo pa kusonkhanitsa kwa chiberekero cha chiberekero cha exfoliated cell (chosakhazikika).
2.Kuzindikira kudzamalizidwa pansi pa kusungidwa kwa masiku a 30 kutentha kozungulira pambuyo pa kusonkhanitsa kwa chiberekero cha chiberekero cha exfoliated cell (chokhazikika).
3.Kuzindikira kudzamalizidwa pansi pa kusungidwa kwa masiku 30 kwa kutentha kozungulira pambuyo pa kusonkhanitsa kwa chitsanzo cha mkodzo;Kuzindikira kudzamalizidwa pakapita nthawi pambuyo potolera zitsanzo zama cell otukuka.
Kayendetsedwe ka magalimoto:200 ma PC / bokosi, 48 ma PC / bokosi.
Zosungirako:2-30 ℃
Nthawi yovomerezeka:12 miyezi
Chipangizo chogwira ntchito:Chida cha Tianlong NP968-C nucleic acid extraction, Tiangen TGuide S96 nucleic acid extraction chida, GENE DIAN EB-1000 nucleic acid extraction chida.
Satifiketi yojambulitsa chipangizo chachipatala No./chofunikira paukadaulo wamankhwala No.:HJXB No. 20210100.
Tsiku lovomerezeka ndi kuwunikiranso malangizo:Tsiku lovomerezeka: Nov. 18, 2021
Zambiri zaife
Monga bizinesi yaukadaulo wapamwamba yomwe idakhazikitsidwa mu 2018 ndi akatswiri apamwamba a epigenetic, Epiprobe imayang'ana kwambiri pakuzindikira kwa khansa ya DNA methylation ndi mafakitale olondola a theranostics.Ndi maziko aukadaulo waukadaulo, tikufuna kutsogolera nthawi yazinthu zatsopano kuti zithetse khansa!
Kutengera kafukufuku wanthawi yayitali wa gulu la Epiprobe, chitukuko ndi kusintha kwa DNA methylation ndi luso lapamwamba kwambiri, kuphatikiza ndi zolinga zapadera za DNA methylation za khansa, timagwiritsa ntchito njira yapadera yophatikizira deta yayikulu ndi ukadaulo wanzeru zopangira. paokha kupanga ukadaulo wapatent-protected liquid biopsy wamadzimadzi.Pofufuza mulingo wa methylation wa malo enaake a zidutswa za DNA zaulere pachitsanzocho, zolephera za njira zowunikira zakale komanso zoletsa za opaleshoni ndi zoyeserera zimapewedwa, zomwe sizimangokwaniritsa kuzindikira kolondola kwa makhansa oyambilira, komanso zimathandizira kuyang'anira nthawi yeniyeni. za zochitika za khansa ndi kukula kwamphamvu.
