Nucleic Acid Extraction Kit (A01)
Mfundo yodziwira
Pambuyo potulutsa DNA ya genomic pogawanitsa maselo okhala ndi lysis buffer, mkanda wa maginito ukhoza kumangirira ku genomic DNA mu zitsanzo.Zonyansa zochepa zomwe zimatengedwa ndi mkanda wa maginito zitha kuchotsedwa ndi chotchinga chotsuka.Mu TE, mkanda wa maginito ukhoza kumasula DNA ya boundgenome, kupeza DNA yapamwamba kwambiri ya genome.Njirayi ndi yophweka komanso yofulumira ndipo khalidwe la DNA lotengedwa ndilopamwamba, lomwe lingakwaniritse zofunikira kuti azindikire DNA methylation.Pakadali pano, zida zopangira zida zotengera mkanda wa maginito zitha kugwirizanirana ndi kutulutsa kwa nucleic acid, kukwaniritsa ntchito zapamwamba kwambiri zochotsa nucleic acid.
Zigawo zazikulu za reagent
Magawo akuwonetsedwa mu tebulo 1:
Table 1 Reagent Zigawo ndi Kutsegula
| Dzina lachigawo | Zigawo zazikulu | Kukula (48) | Kukula (200) |
| 1. Njira ya Lysis | Guanidine hydrochloride, Tris | 11 mL / botolo | 44 ml / botolo |
| 2. Njira zoyeretsera A | NaCl, Tris | 11 mL / botolo | 44 ml / botolo |
| 3. Njira zoyeretsera B | NaCl, Tris | 13 mL / botolo | 26.5mL/botolo *2 |
| 4. Wophunzira | Tris, EDTA | 12 ml / botolo | 44 ml / botolo |
| 5. Protease K solution | Protease K | 1.1mL / chidutswa | 4.4mL / chidutswa |
| 6. Kuyimitsidwa kwa maginito 1 | Maginito mikanda | 1.1mL / chidutswa | 4.4mL / chidutswa |
| 7. Malangizo ochotsa nucleic acid reagents |
| 1 kopi | 1 kopi |
Zomwe zimafunikira pakuchotsa kwa nucleic acid, koma sizinaphatikizidwe mu zida:
1. Reagent: Anhydrous ethanol, isopropanol ndi PBS;
2. Zowonongeka: 50ml centrifuge chubu ndi 1.5ml EP chubu;
3. Zida: Madzi osamba ketulo, pipettor, shelf magnetic, centrifuge, 96-hole deep plate (automatic), automatic nucleic acid extraction equipment (automatic).
Zambiri zoyambira
Zitsanzo zofunika
1. Kuwonongeka kwa mtanda pakati pa zitsanzo kuyenera kupewedwa muzosonkhanitsa ndi kusungirako zitsanzo.
2.Kuzindikiritsa kudzatsirizidwa pansi pa kusungidwa kwa masiku 7 kwa kutentha kozungulira pambuyo posonkhanitsa chiberekero cha chiberekero cha exfoliated cell (chosakhazikika).Kuzindikira kudzamalizidwa pansi pa kusungidwa kwa masiku 30 kwa kutentha kozungulira pambuyo posonkhanitsa chitsanzo cha mkodzo;Kuzindikira kudzamalizidwa pakapita nthawi pambuyo potolera zitsanzo zama cell otukuka.
Kayendetsedwe ka magalimoto:200 ma PC / bokosi, 48 ma PC / bokosi.
Zosungirako:2-30 ℃
Nthawi yovomerezeka:12 miyezi
Chipangizo chogwira ntchito:Chida cha Tianlong NP968-C nucleic acid extraction, Tiangen TGuide S96 nucleic acid extraction chida, GENE DIAN EB-1000 nucleic acid extraction chida.
Satifiketi yojambulitsa chipangizo chachipatala No./chofunikira paukadaulo wamankhwala No.: HJXB No. 20210099.
Tsiku lovomerezeka ndi kusinthidwa kwa malangizo:
Tsiku lovomerezeka: Nov. 18, 2021
