TAGMe DNA Methylation Detection Kits(qPCR) ya Khansa Yachiberekero
NKHANI ZA PRODUCT
Kulondola
Zovomerezeka pa zitsanzo zachipatala za 36000 m'maphunziro apakati akhungu amitundu yambiri, mankhwalawo ali ndi 94.3% ndi kukhudzika kwa 96.0%.
Zosavuta
Tekinoloje yoyambirira ya Me-qPCR yozindikira methylation imatha kumalizidwa mu sitepe imodzi mkati mwa maola atatu popanda kusintha kwa bisulfite.
Kumayambiriro
Kuyezetsa khansa ya pachibelekeropo kumatha kupititsidwa patsogolo mpaka siteji ya zotupa zapamwamba (zotupa za precancerous).
Zochita zokha
Kuphatikizidwa ndi pulogalamu yowunikira zotsatira, kutanthauzira kwazotsatira kumangochitika zokha komanso kuwerengeka mwachindunji.
ZOYENERA KUGWIRITSA NTCHITO
Chidachi chimagwiritsidwa ntchito mu vitro qualitative kuzindikira kwa hypermethylation ya jini PCDHGB7 mu zitsanzo za khomo lachiberekero.Zotsatira zabwino zimasonyeza chiopsezo chowonjezereka cha kalasi ya 2 kapena yapamwamba / yapamwamba kwambiri ya khomo lachiberekero intraepithelial neoplasia (CIN2 +, kuphatikizapo CIN2, CIN3, adenocarcinoma in situ, ndi khansa ya pachibelekero), yomwe imafuna colposcopy ndi / kapena histopathological kufufuza.M'malo mwake, zotsatira zoyipa zoyesa zikuwonetsa kuti chiwopsezo cha CIN2 + ndi chochepa, koma chiwopsezocho sichingachotsedwe kwathunthu.Kuzindikira komaliza kuyenera kutengera colposcopy ndi/kapena histopathological zotsatira.PCDHGB7 ndi membala wa banja la protocadherin γ gene cluster.Protocadherin yapezeka kuti imayang'anira njira zachilengedwe monga kuchuluka kwa ma cell, kuzungulira kwa ma cell, apoptosis, kuwukira, kusamuka komanso autophagy ya maselo otupa kudzera m'njira zosiyanasiyana zowonetsera, komanso kutonthola kwake kwa majini chifukwa cha hypermethylation ya dera lolimbikitsa kumagwirizana kwambiri ndi zomwe zimachitika komanso chitukuko. za khansa zambiri.Zanenedwa kuti hypermethylation ya PCDHGB7 imagwirizanitsidwa ndi zotupa zosiyanasiyana, monga non-Hodgkin lymphoma, khansa ya m'mawere, khansa ya chiberekero, khansa ya endometrial ndi khansa ya chikhodzodzo.
MFUNDO YODZIWA
Chidachi chili ndi nucleic acid extraction reagent ndi PCR kuzindikira reagent.Nucleic acid imachotsedwa ndi maginito-based based method.Chidachi chimachokera pa mfundo ya njira ya fluorescence quantitative PCR, pogwiritsa ntchito methylation-specific real-time PCR reaction kuti ifufuze template ya DNA, ndi nthawi yomweyo kuzindikira malo a CpG a PCDHGB7 jini ndi chizindikiro chowongolera khalidwe lamkati lamtundu wa G1 ndi G2.Mulingo wa methylation wa PCDHGB7 pachitsanzo, kapena mtengo wa Me, umawerengedwa molingana ndi PCDHGB7 gene methylated DNA amplification Ct value ndi Ct value of the reference.PCDHGB7 jini hypermethylation zabwino kapena zoipa zimatsimikiziridwa molingana ndi mtengo wa Me.
Zochitika zantchito
Kuwunika koyambirira
Anthu athanzi
Kuwunika Kuopsa kwa Khansa
Chiwerengero cha anthu omwe ali pachiwopsezo chachikulu (omwe ali pachiwopsezo chachikulu cha papillomavirus yamunthu (hrHPV) kapena zabwino za cervical exfoliation cytology)
Kubwereza Zobwereza
Chiwerengero cha postoperative (omwe ali ndi mbiri ya zilonda zam'mimba zapamwamba kapena khansa ya khomo lachiberekero)
Kufunika kwachipatala
Kuwunika koyambirira kwa anthu athanzi:Khansara ya khomo lachiberekero ndi zotupa za precancerous zitha kuyesedwa molondola
Kuwunika kwachiwopsezo mwa anthu omwe ali pachiwopsezo chachikulu:Gulu lachiwopsezo litha kuchitidwa m'magulu omwe ali ndi HPV kuti awongolere kuwunika kotsatira
Kuyang'anira mobwerezabwereza kwa chiwerengero cha anthu ochita opaleshoni:Kuwunika kwa chiwerengero cha anthu pambuyo pa opaleshoni kungathe kuchitidwa pofuna kupewa kuchedwa kwa chithandizo chifukwa cha kubwereza
Zosonkhanitsa zitsanzo
Njira yachitsanzo: Ikani chitsanzo cha khomo pachibelekeropo, pakani pang'onopang'ono burashi ya khomo lachiberekero ndikuzungulira nthawi 4-5 molunjika, pang'onopang'ono chotsani burashi ya khomo lachiberekero, ikani mu njira yosungira ma cell, ndikulembera kuti mufufuze.
Kusungidwa kwa zitsanzo:Zitsanzo zimatha kusungidwa kutentha kwa masiku 14, pa 2-8 ℃ kwa miyezi iwiri, ndi -20 ± 5 ℃ kwa miyezi 24.
Njira yodziwira: Maola a 3 (popanda ndondomeko yamanja)
DNA Methylation Detection Kits (qPCR) ya Khansa ya Urothelial
| Kugwiritsa ntchito kuchipatala | Clinical wothandiza matenda a khansa ya khomo pachibelekeropo |
| Jini yozindikira | PCDHGB7 |
| Mtundu wachitsanzo | Zitsanzo za khomo lachiberekero lachikazi |
| Njira yoyesera | Fluorescence kuchuluka kwa PCR luso |
| Ntchito chitsanzo | ABI7500 |
| Kufotokozera kwapaketi | 48 mayeso / zida |
| Zosungirako | Kit A iyenera kusungidwa pa 2-30 ℃ Zida B ziyenera kusungidwa pa -20±5 ℃ Imagwira ntchito mpaka miyezi 12 |
