TAGMe DNA Methylation Detection Kits (qPCR) ya Khansa ya Urothelial
NKHANI ZA PRODUCT
Kulondola
Zovomerezeka pa zitsanzo zachipatala za 3500 m'maphunziro apakati-akhungu amitundu yambiri, mankhwalawo ali ndi 92.7% ndi chidziwitso cha 82.1%.
Zosavuta
Tekinoloje yoyambirira ya Me-qPCR yozindikira methylation imatha kumalizidwa mu sitepe imodzi mkati mwa maola atatu popanda kusintha kwa bisulfite.
Zosasokoneza
Ndi mamililita 30 okha a mkodzo omwe amafunikira kuti azindikire mitundu itatu ya khansa, kuphatikizapo khansa ya m'chiuno, khansa ya mkodzo, khansa ya chikhodzodzo nthawi imodzi.
Zochitika zantchito
Matenda Othandizira
Chiwerengero cha anthu omwe akudwala hematuria yopanda ululu / omwe akuganiziridwa kuti ali ndi urothelial (khansa ya ureter / khansa ya m'chiuno)
Kuwunika Kuopsa kwa Khansa
Opaleshoni / chemotherapy-ofuna anthu okhala ndi urothelial carcinoma;
Kubwereza Zobwereza
Chiwerengero cha postoperative ndi urothelial carcinoma
ZOYENERA KUGWIRITSA NTCHITO
Chidachi chimagwiritsidwa ntchito pozindikira mu vitro qualitative kuzindikira kwa hypermethylation ya jini ya Urothelial Carcinoma (UC) m'zitsanzo za urothelial.Zotsatira zabwino zikuwonetsa chiwopsezo chowonjezereka cha UC, chomwe chimafunikira cystoscope yowonjezereka komanso / kapena kuwunika kwa histopathological.M'malo mwake, zotsatira zoyipa zoyesa zikuwonetsa kuti chiwopsezo cha UC ndi chochepa, koma chiwopsezocho sichingachotsedwe kwathunthu.Kuzindikira komaliza kuyenera kutengera cystoscope ndi/kapena histopathological zotsatira.
MFUNDO YODZIWA
Chidachi chili ndi nucleic acid extraction reagent ndi PCR kuzindikira reagent.Nucleic acid imachotsedwa ndi maginito-based based method.Chidachi chimachokera pa mfundo ya njira ya fluorescence quantitative PCR, pogwiritsa ntchito methylation-specific real-time PCR reaction kuti ifufuze template ya DNA, ndikuwonanso malo a CpG a UC gene ndi chizindikiro chowongolera khalidwe lamkati la jini ya G1 ndi G2.Mulingo wa methylation wa UC gene, wotchedwa Me value, umawerengedwa molingana ndi UC gene methylated DNA amplification Ct value ndi Ct value of the reference.UC gene hypermethylation zabwino kapena zoipa zimatsimikiziridwa molingana ndi mtengo wa Me.
DNA Methylation Detection Kits (qPCR) ya Khansa ya Urothelial
| Kugwiritsa ntchito kuchipatala | chipatala wothandiza matenda a urothelial cnacer;kuwunika momwe chithandizo chamankhwala chimagwirira ntchito / chemotheray;kuwunika kubwereza kwa postoperative |
| Jini yozindikira | UC |
| Mtundu wachitsanzo | Zitsanzo za cell za mkodzo (sediment ya mkodzo) |
| Njira yoyesera | Fluorescence kuchuluka kwa PCR luso |
| Zitsanzo zoyenera | ABI7500 |
| Kufotokozera kwapaketi | 48 mayeso / zida |
| Zosungirako | Kit A iyenera kusungidwa pa 2-30 ℃ Zida B ziyenera kusungidwa pa -20±5 ℃ Imagwira ntchito mpaka miyezi 12. |
