TAGMe DNA Methylation Detection Kits (qPCR) ya Khansa ya Endometrial
NKHANI ZA PRODUCT
Kulondola
Zovomerezeka pa zitsanzo zachipatala za 800 m'maphunziro apakati akhungu amitundu yambiri, mankhwalawa ali ndi 82.81% ndi chidziwitso cha 80.65%.
Zosavuta
Tekinoloje yoyambirira ya Me-qPCR yozindikira methylation imatha kumalizidwa mu sitepe imodzi mkati mwa maola atatu popanda kusintha kwa bisulfite.
Kumayambiriro
Kuzindikirika pa precancerous stage.
Zochita zokha
Itha kugwiritsidwa ntchito ndi burashi ya khomo lachiberekero ndi zitsanzo za Pap smear.
ZOYENERA KUGWIRITSA NTCHITO
Chidachi chimagwiritsidwa ntchito mu vitro qualitative kuzindikira kwa hypermethylation ya jini PCDHGB7 incervical specimens.Zotsatira zabwino zikuwonetsa chiwopsezo chowonjezereka cha zotupa zam'mimba zam'mimba ndi khansa, zomwe zimafunikira kuwunika kwa histopathological kwa endometrium.M'malo mwake, zotsatira zoyipa zoyesa zikuwonetsa kuti chiopsezo cha zilonda zam'mimba zam'mimba ndi khansa ndizochepa, koma chiopsezo sichingasiyidwe kwathunthu.Chomaliza matenda ayenera zochokera histopathological kufufuza zotsatira za endometrium.PCDHGB7 ndi membala wa banja la protocadherin γ gene cluster.Protocadherin yapezeka kuti imayang'anira njira zachilengedwe monga kuchuluka kwa ma cell, kuzungulira kwa ma cell, apoptosis, kuwukira, kusamuka komanso autophagy ya maselo otupa kudzera m'njira zosiyanasiyana zowonetsera, komanso kutonthola kwake kwa majini chifukwa cha hypermethylation ya dera lolimbikitsa kumagwirizana kwambiri ndi zomwe zimachitika komanso chitukuko. za khansa zambiri.Zanenedwa kuti hypermethylation ya PCDHGB7 imagwirizanitsidwa ndi zotupa zosiyanasiyana, monga non-Hodgkin lymphoma, khansa ya m'mawere, khansa ya chiberekero, khansa ya endometrial ndi khansa ya chikhodzodzo.
MFUNDO YODZIWA
Chidachi chili ndi nucleic acid extraction reagent ndi PCR kuzindikira reagent.Nucleic acid imachotsedwa ndi maginito-based based method.Chidachi chimachokera pa mfundo ya njira ya fluorescence quantitative PCR, pogwiritsa ntchito methylation-specific real-time PCR reaction kuti ifufuze template ya DNA, ndi nthawi yomweyo kuzindikira malo a CpG a PCDHGB7 jini ndi chizindikiro chowongolera khalidwe lamkati lamtundu wa G1 ndi G2.Mulingo wa methylation wa PCDHGB7 pachitsanzo, kapena mtengo wa Me, umawerengedwa molingana ndi PCDHGB7 gene methylated DNA amplification Ct value ndi Ct value of the reference.PCDHGB7 jini hypermethylation zabwino kapena zoipa zimatsimikiziridwa molingana ndi mtengo wa Me.
Zochitika zantchito
Kuwunika koyambirira
Anthu athanzi
Kuwunika Kuopsa kwa Khansa
Magulu omwe ali pachiwopsezo chachikulu (anthu omwe amatuluka magazi osadziwika bwino pambuyo posiya kusamba, kuchuluka kwa endometrial, etc.)
Kubwereza Zobwereza
Chiwerengero cha anthu
Kufunika kwachipatala
Kuwunika koyambirira kwa anthu athanzi:Khansara ya endometrial ndi zotupa za precancerous zikhoza kuyesedwa molondola;
Kuwunika kwachiwopsezo kwa anthu omwe ali pachiwopsezo chachikulu:Kuwunika kwachiwopsezo kungathe kuchitidwa kwa anthu omwe ali ndi magazi osadziwika bwino mu nyini ndi kukhuthala kwa endometrial pambuyo posiya kusamba kuti athandizire kuzindikira matenda;
Kuwunika kwachiwerengero cha anthu:Kuwunika kwa chiwerengero cha anthu pambuyo pa opaleshoni kungathe kuchitidwa pofuna kupewa kuchedwa kwa chithandizo chifukwa cha kubwereza.
Zosonkhanitsa zitsanzo
Njira yachitsanzo: Ikani chitsanzo cha khomo pachibelekeropo, pakani pang'onopang'ono burashi ya khomo lachiberekero ndikuzungulira nthawi 4-5 molunjika, pang'onopang'ono chotsani burashi ya khomo lachiberekero, ikani mu njira yosungira ma cell, ndikulembera kuti mufufuze.
Kusungidwa kwa zitsanzo:Zitsanzo zimatha kusungidwa kutentha kwa masiku 14, pa 2-8 ℃ kwa miyezi iwiri, ndi -20 ± 5 ℃ kwa miyezi 24.
Njira yodziwira: Maola a 3 (popanda ndondomeko yamanja)
TAGMe DNA Methylation Detection Kits (qPCR) ya Khansa ya Endometrial
| Kugwiritsa ntchito kuchipatala | Kuzindikira kothandiza kwachipatala kwa endometrial carcinoma |
| Jini yozindikira | PCDHGB7 |
| Mtundu wachitsanzo | Zitsanzo za khomo lachiberekero lachikazi |
| Njira yoyesera | Fluorescence kuchuluka kwa PCR luso |
| Zitsanzo zoyenera | ABI7500 |
| Kufotokozera kwapaketi | 48 mayeso / zida |
| Zosungirako | Kit A iyenera kusungidwa pa 2-30 ℃ Zida B ziyenera kusungidwa pa -20±5 ℃ Imagwira ntchito mpaka miyezi 12. |
Zambiri zaife
Epiprobe ili ndi zomangamanga zonse: malo opangira GMP ali ndi malo a 2200 square metres, ndikusunga dongosolo la ISO13485, lomwe limakwaniritsa zofunikira zopanga mitundu yonse yazinthu zoyesa chibadwa;labotale yachipatala imakhala ndi malo a 5400 masikweya mita ndipo imatha kuchita bizinesi yozindikira khansa ya methylation ngati labotale yovomerezeka ya chipani chachitatu.Kupatula apo, tili ndi zinthu zitatu zomwe zidalandira satifiketi ya CE, zophimba khansa ya khomo lachiberekero, khansa ya endometrial komanso kuzindikira kokhudzana ndi khansa ya urothelial.
Ukadaulo wozindikira khansa ya Epiprobe ungagwiritsidwe ntchito powunika khansa yoyambirira, kuwunika kothandizira, kuwunika kwapambuyo komanso kuwunika kwapambuyo, kuwunika kwa recrudescence, komwe kumadutsa njira yonse ya matenda a khansa ndi chithandizo, kupereka mayankho abwino kwa madokotala ndi odwala.
