TAGMe DNA Methylation Detection Kits (qPCR) ya Cervical Cancer / Endometrial Cancer
NKHANI ZA PRODUCT
Zosasokoneza
Itha kugwiritsidwa ntchito ndi burashi ya khomo lachiberekero ndi zitsanzo za Pap smear.
Zosavuta
Tekinoloje yoyambirira ya Me-qPCR yozindikira methylation imatha kumalizidwa mu sitepe imodzi mkati mwa maola atatu popanda kusintha kwa bisulfite.
Kumayambiriro
Kuzindikirika pa precancerous stage.
Zochita zokha
Kuphatikizidwa ndi pulogalamu yowunikira zotsatira, kutanthauzira kwazotsatira kumangochitika zokha komanso kuwerengeka mwachindunji.
Zochitika zantchito
Kuwunika koyambirira
Anthu athanzi
Kuwunika Kuopsa kwa Khansa
Chiwerengero cha anthu omwe ali pachiwopsezo chachikulu (omwe ali pachiwopsezo chachikulu cha papillomavirus yamunthu (hrHPV) kapena ali pachiwopsezo cha cervical exfoliation cytology / positive papillomavirus yapapillomavirus yamunthu (hrHPV) kapena cytology yotulutsa khomo lachiberekero)
Kubwereza Zobwereza
Chiwerengero cha anthu
ZOYENERA KUGWIRITSA NTCHITO
Chidachi chimagwiritsidwa ntchito mu vitro qualitative kuzindikira kwa hypermethylation ya jini PCDHGB7 mu zitsanzo za khomo lachiberekero.Kwa khansa ya khomo lachiberekero, zotsatira zabwino zimasonyeza chiopsezo chowonjezeka cha kalasi 2 kapena apamwamba / apamwamba kwambiri a khomo lachiberekero intraepithelial neoplasia (CIN2 +, kuphatikizapo CIN2, CIN3, adenocarcinoma in situ, ndi khansa ya khomo lachiberekero), yomwe imafuna colposcopy ndi / kapena histopathological kufufuza. .M'malo mwake, zotsatira zoyipa zoyesa zikuwonetsa kuti chiwopsezo cha CIN2 + ndi chochepa, koma chiwopsezocho sichingachotsedwe kwathunthu.Kuzindikira komaliza kuyenera kutengera colposcopy ndi/kapena histopathological zotsatira.Komanso, chifukwa cha khansa ya endometrial, zotsatira zabwino zimasonyeza chiopsezo chowonjezeka cha zotupa za endometrial precancerous ndi khansa, zomwe zimafunika kufufuza kwa histopathological kwa endometrium.M'malo mwake, zotsatira zoyipa zoyesa zikuwonetsa kuti chiopsezo cha zilonda zam'mimba zam'mimba ndi khansa ndizochepa, koma chiopsezo sichingasiyidwe kwathunthu.Chomaliza matenda ayenera zochokera histopathological kufufuza zotsatira za endometrium.
PCDHGB7 ndi membala wa banja la protocadherin γ gene cluster.Protocadherin yapezeka kuti imayang'anira njira zachilengedwe monga kuchuluka kwa ma cell, kuzungulira kwa ma cell, apoptosis, kuwukira, kusamuka komanso autophagy ya maselo otupa kudzera m'njira zosiyanasiyana zowonetsera, komanso kutonthola kwake kwa majini chifukwa cha hypermethylation ya dera lolimbikitsa kumagwirizana kwambiri ndi zomwe zimachitika komanso chitukuko. za khansa zambiri.Zanenedwa kuti hypermethylation ya PCDHGB7 imagwirizanitsidwa ndi zotupa zosiyanasiyana, monga non-Hodgkin lymphoma, khansa ya m'mawere, khansa ya chiberekero, khansa ya endometrial ndi khansa ya chikhodzodzo.
MFUNDO YODZIWA
Chidachi chili ndi nucleic acid extraction reagent ndi PCR kuzindikira reagent.Nucleic acid imachotsedwa ndi maginito-based based method.Chidachi chimachokera pa mfundo ya njira ya fluorescence quantitative PCR, pogwiritsa ntchito methylation-specific real-time PCR reaction kuti ifufuze template ya DNA, ndi nthawi yomweyo kuzindikira malo a CpG a PCDHGB7 jini ndi chizindikiro chowongolera khalidwe lamkati lamtundu wa G1 ndi G2.Mulingo wa methylation wa PCDHGB7 pachitsanzo, kapena mtengo wa Me, umawerengedwa molingana ndi PCDHGB7 gene methylated DNA amplification Ct value ndi Ct value of the reference.PCDHGB7 jini hypermethylation zabwino kapena zoipa zimatsimikiziridwa molingana ndi mtengo wa Me.
