Gargle Nucleic Acid Extraction Reagents
MFUNDO YODZIWA
Zogulitsazo makamaka zimakhala ndi gargle, osakaniza kwambiri absorbant superparamagnetic nanospheres, ndi ma lysis reagents apadera.Mikanda yapadera yamaginito imakhala ndi mgwirizano wabwino pazigawo zakuthupi (kuphatikiza ma virus aulere ndi ma cell omwe ali ndi kachilomboka).Mukalumikizana ndi yankho la lysis, osakhala ndi ionic cell / nucleus-membrane-breaking surfactants ndi protease inhibitors mu yankho amatha kuletsa ntchito ya DNA / RNA enzyme, ndikukhazikitsa nucleic acid.Zinthu zonse za nucleic acid mu zigawo za gargle zimatulutsidwa bwino mu njira ya lysis, kupeza nucleic acid mwachangu.Zitsanzo za gargle zomwe zimagwiritsa ntchito zidazi sizifuna kuyeretsedwa kwa nucleic acid, zomwe zitha kugwira ntchito mwachindunji ku zida zodziwira za nucleic acid.
Zigawo zazikulu za reagent
Magawo akuwonetsedwa mu tebulo 1.
Table 1 Zigawo ndi Kutsegula mu Kit
| Dzina lachigawo | Zigawo zazikulu | Kukula (1) | Kukula (10) | Kukula (30) | Kukula (50) |
| 1. Gargle A | NaCl | 8mL / chubu | 8mL/chubu *10 machubu | 8mL/chubu *30 machubu | 8mL/chubu *50 machubu |
| 2. Wosonkhanitsa Gargle | PP | 1 chidutswa | 10 ma PC | 30 ma PC | 50 ma PC |
| 3. Njira Yowonjezera B | Maginito mikanda | 2 ml / chubu | 2mL/chubu *10 machubu | 2mL/chubu *30 machubu | 2mL/chubu *50 machubu |
| 4 Lysis Buffer C | Protease K | 0.2mL / chidutswa | 0.2mL / chidutswa * 10 ma PC | 0.2mL / chidutswa * 30 ma PC | 0.2mL / chidutswa * 50 ma PC |
| 5. Maginito Cap | Magnet | 1 chidutswa | 10 ma PC | 30 ma PC | 50 ma PC |
Zidazo ndizovomerezeka kwa miyezi 12.
Zomwe zimafunikira pakuchotsa kwa nucleic acid, koma sizinaphatikizidwe mu zida:
1. Zowonongeka: 1.5ml EP chubu;
2. Zida: Kusamba madzi (kapena kusamba kwachitsulo), pipettes, ndi centrifuge.
Zambiri zoyambira
Zitsanzo zofunika:
1. Kugwiritsidwa ntchito kwa nucleic acid m'zigawo ndi kulemeretsa zitsanzo za gargle.
2. Chitsanzo cha gargle chidzawonjezedwa ku njira yowonjezera B mu nthawi yosonkhanitsa.Mikanda ya maginito yotengedwa idzasamutsidwa ku lysis buffer C nthawi yomweyo.Zitsanzo zomwe zinawonjezeredwa ku lysis buffer C zikhoza kusungidwa kutentha.
Kapangidwe kazonyamula: 1 chidutswa / bokosi, 10 ma PC / bokosi, 30 ma PC / bokosi, ndi 50 ma PC / bokosi.
Zosungirako: Njira yowonjezera B ndi lysis solution C iyenera kusungidwa kwa miyezi 12 pa 2-8 ℃, ndi zigawo zina zikhoza kusungidwa kwa miyezi 12 pansi pa kutentha kwa chipinda;Chidacho chikhoza kunyamulidwa ndikusungidwa kwakanthawi pansi pa kutentha kozungulira, komwe sikungapitirire masiku 5.
Nthawi yovomerezeka: 12 miyezi
Satifiketi yojambulitsa chipangizo chachipatala No./chofunikira paukadaulo wamankhwala No.:HJXB No. 20220086.
Tsiku lovomerezeka ndi kuwunikiranso malangizowa:
Tsiku lovomerezeka: Oct. 26, 2022
